Since the introduction of Huánglóngbìng (HLB—yellow dragon disease—better known as citrus greening disease) onto U.S. soil in a Florida citrus grove in 2005, the disease has been a major threat to commercial citrus production across the country.
Before arriving in North America, HLB had already carved a path of destruction across the Far East, Africa, the Indian subcontinent and the Arabian Peninsula, and was discovered in July 2004 in Brazil. In its wake it left citrus growers around the world astounded at the inevitable and long-lasting risks the disease poses to the global citrus industry.
During the first two years after reaching Florida, the disease destroyed a huge section of the state’s valuable citrus industry, and by 2009, just five years after its introduction in the region, almost every county within Florida had confirmed HLB cases, including commercial and private citrus groves. From there, the disease spread to adjoining states, eventually reaching citrus growing areas in Texas and finally as far west as California.
The fight against HLB and the tiny psyllids that carry the bacteria from tree to tree is about as old as the disease itself. Recognizing the disease had the ability to threaten the global citrus industry, researchers from around the world began working on possible solutions to combat the spread of this dangerous citrus killer.
In spite of early efforts however, the tell-tale signs of the disease kept spreading.
The early symptoms of HLB include leaves with yellowing veins appearing along with asymmetrical chlorosis referred to as “blotchy mottle.” These are the most diagnostic symptoms of the disease, especially on sweet orange. Growers, ever fearful the disease would reach their trees, have been on constant lookout for leaves that are slow to develop and often with a variety of chlorotic patterns that often resemble mineral deficiencies such as zinc, iron, and manganese.
Regardless of treatment efforts, once established in a grove, the end result of the disease is proving to be inevitable, the complete decay and destruction of all infected trees.
Detection is one of the first hurdles. Growers face a number of unique challenges. For one, HLB-infected citrus trees do not show symptoms during the first year of infection, so there is a long period of time when a grower cannot visually detect an infected tree. But that hasn’t stemmed research efforts.
The spreading pandemic of the disease served to rally the global citrus industry and the many researchers who support it. Soon new and innovative treatments were being tested. In addition to antibacterial management and control, and management of the psyllids that carry the disease, tree removal became a standard procedure to help curtail the rapid spread of the bacterium.
Soon, beneficial parasitoids were introduced and widely used to help control psyllid populations. Heat treatments in nurseries and on field trees covered by plastic wrap offered some success in slowing the disease process in early research efforts. Researchers spent hundreds of millions of dollars worldwide searching for a cure. A zinc-based bactericidal spray seemed to offer some hope.
Breeders offered new citrus varieties resistant to the bacterium that causes HLB. Bio-engineers have been devising methods to make citrus trees less attractive to the psyllids that carry the disease. But in recent months a new idea has surfaced, and while no one is ringing the bell of victory, researchers on the project are quietly voicing new hope in the war against the disease.
HOW IT WORKS
According to researchers, the reproductive and feeding habits of the psyllid make it the perfect carrier of the bacterium. An infected psyllid creates a localized infection when it feeds and transmits the bacterium into a citrus tree. It does not take long for the bacterium to spread throughout the plant, but the inoculum is first concentrated in the leaves and stems where the infected psyllid feeds. Female psyllids lay eggs in the same region where they feed. If these females are infected, their nymphs, which begin feeding in the infected area of the tree when they hatch, eventually acquire the bacterium, molt to the winged adult stage and disperse taking the bacterium along with them.
Researchers at the Boyce Thompson Institute, a premier life sciences research institution located in Ithaca, New York, on the Cornell University campus, have concentrated recent efforts on the psyllid itself as a possible link to control.
Michelle Cilia, a research molecular biologist at the USDA Agricultural Research Service and assistant professor at the Boyce Thompson Institute (BTI), and her team of researchers have been looking at a protein that makes the bellies of citrus psyllids blue and studying the possible connection it may have with the natural process of spreading the devastating bacterium in the first place. Researchers say Asian citrus psyllids with blue abdomens have high levels of an oxygen-transporting protein called hemocyanin.